Journal: Medical Science Monitor Basic Research

Article Title: Reprogramming Bone Marrow Stem Cells to Functional Endothelial Cells in a Mini Pig Animal Model

doi: 10.12659/MSMBR.905081

Figure Lengend Snippet: Angiogenesis and reprogrammed bone marrow stem cells (BMSCs). ( A ) Representative images of angiogenesis seen in reprogrammed bone marrow stem cells (BMSCs), arterial endothelial cells (AEC), and venous endothelial cells (VEC) but not in normal BMSCs. ( B ) Quantitative investigation of angiogenesis using a grading system: Grade 0=no angiogenesis, to Grade 5=‘mesh-like’ branching structures. * p≤0.05 when compared with normal BMSCs.

Article Snippet: An in vitro angiogenesis assay kit was used (Merk Millipore, Berlin, Germany) using the method previously described [ ].

Techniques:

Journal: Scientific Reports

Article Title: Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro

doi: 10.1038/s41598-018-30446-1

Figure Lengend Snippet: Effects on tube formation in HUVECs treated with exosomes released from PK-45H cells. Tube formation assay was performed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Cosmo Bio). A dynamin-dependent exosomal endocytosis promoted tube formation in HUVECs. Data are expressed as means ± standard deviations (each n = 3). Statistical significance ( P ≤ 0.05) was examined with the Tukey–Kramer test for multiple comparisons.

Article Snippet: Tube formation assay was performed using the Cultrex In Vitro Angiogenesis Assay Tube Formation Kit (Cosmo Bio).

Techniques: Tube Formation Assay, In Vitro, Angiogenesis Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: 10-Hydroxy-2-decenoic Acid, a Major Fatty Acid from Royal Jelly, Inhibits VEGF-induced Angiogenesis in Human Umbilical Vein Endothelial Cells

doi: 10.1093/ecam/nem152

Figure Lengend Snippet: 10HDA and MMP inhibitor prevented in vitro tube formation in HUVECs. ( A ) In vitro tube formation was achieved using an in vitro angiogenesis kit. Briefly, HUVECs and fibroblasts were incubated with VEGF (10 ng/ml) (b–f), together with 10HDA (20 µM) (c), 10HDA (100 µM) (d), 10HDA (500 µM) (e), or GM6001 (10 µM) (f). Then 11 days afterwards, they were stained with anti-CD31 antibody, an endothelial cell marker. Control is shown in (a). Scale bar represents 1 mm. ( B ) Tube formation was evaluated by measurements of joints, path, tube length and tube area, as described in ‘Methods’. Data represent means and standard error ( n = 5). *** P < 0.01 vs. control; * P < 0.05, ** P < 0.01 vs. vehicle (Student's t -test).

Article Snippet: To evaluate tube formation by endothelial cells, a HUVECs in vitro angiogenesis kit (Kurabo) was used.

Techniques: In Vitro, Incubation, Staining, Marker, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: 10-Hydroxy-2-decenoic Acid, a Major Fatty Acid from Royal Jelly, Inhibits VEGF-induced Angiogenesis in Human Umbilical Vein Endothelial Cells

doi: 10.1093/ecam/nem152

Figure Lengend Snippet: 10HDA prevented VEGF-induced cell proliferation in HUVECs. HUVECs were incubated with the indicated concentrations of 10HDA in the presence or absence of VEGF (10 ng/ml) for 3 days at 37°C in 5% CO 2 with humidity. Cell proliferation was estimated using a CCK-8. VEGF treatments increased cell viability 2-fold (vs. control). 10HDA at 500 µM had no effect by itself, yet inhibited VEGF-induced proliferation. Data represent means and standard error ( n = 6). *** P < 0.01 vs. control, ** P < 0.01 vs. vehicle (Student's t -test).

Article Snippet: To evaluate tube formation by endothelial cells, a HUVECs in vitro angiogenesis kit (Kurabo) was used.

Techniques: Incubation, CCK-8 Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: 10-Hydroxy-2-decenoic Acid, a Major Fatty Acid from Royal Jelly, Inhibits VEGF-induced Angiogenesis in Human Umbilical Vein Endothelial Cells

doi: 10.1093/ecam/nem152

Figure Lengend Snippet: 10HDA suppressed cell migration on in vitro wound-healing assay. HUVECs migration was assessed using a wound-healing assay. Briefly, 90% confluent monolayers of HUVECs were scratch wounded, and then incubated for 24 h. ( A ) Images of wounded monolayer of HUVECs taken at times 0 h and 24 h after treatment with VEGF (10 ng/ml) with or without 10HDA (500 µM) or GM6001 (10 µM). The horizontal lines indicate the wound edge. Wound closure in response to VEGF stimulation was reduced by addition of 10HDA or GM6001. Scale bar represents 500 µm. ( B ) Migration was estimated by measurement of cell numbers within the wounded region. 10HDA and GM6001 had no effects by themselves, yet decreased VEGF-induced migration (vs. vehicle). Data represent means and standard error ( n = 6). *** P < 0.01 vs. control, ** P < 0.01 vs. vehicle (Student's t -test).

Article Snippet: To evaluate tube formation by endothelial cells, a HUVECs in vitro angiogenesis kit (Kurabo) was used.

Techniques: Migration, In Vitro, Wound Healing Assay, Incubation, Control